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Molecular basis to the enzymatic inactivity of class III glutaredoxin ROXY9 on common glutathionylated substrates

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, Just about no information and facts is available for class III GRXs. This has actually been due to encountered troubles when purifying recombinant proteins expressed in E. coli30. Below, we succeeded in getting milligram amounts of course III GRX ROXY9 from Arabidopsis thaliana by implementing the baculovirus expression process in insect cells.

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The colour code in the triangles corresponds into the colour code on the redox state as determined by mass spectrometry. Molecular masses of marker proteins (M) are indicated in kDa. (b, file) Relative intensity proportions of peptides that contains the Lively website While using the indicated modifications. The outcome are from a few or four replicates, with Every single replicate representing an impartial procedure. Source facts are supplied for a Source Facts file.